840:153g:Projects/project27/2012/11/08: Difference between revisions

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* A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets.
* A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets.
[[Image:MmoPCR1.jpg|400px]]
[[Image:MmoPCR1.jpg]]


* No distinct bands were shown when PCR products were run on gel electrophoresis.  This indicates that the target gene fragments were not amplified significantly.
* No distinct bands were shown when PCR products were run on gel electrophoresis.  This indicates that the target gene fragments were not amplified significantly.

Revision as of 14:29, 27 November 2012

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Failed Transformation

  • The transformation from 11/6 showed no usable results. Colonies of positive control cells were present, grown on ampicillin media. Colonies were also present of negative control cells (transformed with water) and untransformed cells that were grown on media not containing antibiotics. These results indicate that the transformation was performed correctly, but that the target plasmid-promoter complex was not appropriately transformed into the competent cells
  • A PCR was performed according to the protocol designed on 11/6, using M. trichosporium genomic DNA as template, and both primer sets.

  • No distinct bands were shown when PCR products were run on gel electrophoresis. This indicates that the target gene fragments were not amplified significantly.
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