840:153g:Projects/project27/2012/11/08: Difference between revisions
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* A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets. | * A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets. | ||
* | * A ligation of the J23100 promoter and each of the two target vectors, pSB1K3 and J61002, was again attempted. This was performed at 4°C to test whether ligation temperature would increase the rate of successful ligation and therefore lead to the transformation of a functional plasmid-insert complex into ''E. coli''. | ||
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Revision as of 15:26, 27 November 2012
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Failed Transformation
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