840:153g:Projects/project27/2012/10/16: Difference between revisions
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==Plasmid Backbone Isolation== | ==Plasmid Backbone Isolation== | ||
* The pSB1K3 and J61002 plasmids were digested with Xba1 and Pst1 enzymes in order to remove inserted parts. pSB1K3 contained a 1022bp insert including parts R0010 (Lac1), B0034, E1010 (mRFP1), and B0015. J61002 contained a ~845bp insert including J23100, E1010 (mRFP1), and B0015. Digested plasmid DNA was run on a gel to separate plasmid backbone from insert DNA. Gel bands containing plasmid backbone were excised and stored for DNA recovery. | * The pSB1K3 and J61002 plasmids were digested with Xba1 and Pst1 enzymes in order to remove inserted parts. pSB1K3 contained a 1022bp insert including parts R0010 (Lac1), B0034, E1010 (mRFP1), and B0015. J61002 contained a ~845bp insert including J23100, E1010 (mRFP1), and B0015. Digested plasmid DNA was run on a gel to separate plasmid backbone from insert DNA. Gel bands containing plasmid backbone were excised and stored for DNA recovery. | ||
[[Image: | [[Image:Digest3-2.jpg]] | ||
Revision as of 19:35, 18 October 2012
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Plasmid Backbone Isolation
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