840:153g:Projects/project27/2012/09/20: Difference between revisions
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(Autocreate 2012/09/20 Entry for 840:153g:Projects/project27) |
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==Entry title== | ==Entry title== | ||
* | * Transformed chemically competent ''E. coli'' cells with pSB1A3 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control) | ||
* After transformation, cultures were plated in the following quantities on antibiotic-containing media: | |||
- pSB1A3(& J23100): 200ul on ampicillin | |||
- pSB1K3: 200ul on kanamycin | |||
- pSB1A7: 50ul on ampicillin | |||
50ul on kanamycin | |||
50ul on no antibiotic | |||
- dH2O: 50ul on ampicillin | |||
50ul on kanamycin | |||
50ul on no antibiotic | |||
Revision as of 14:05, 20 September 2012
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Entry title
- pSB1A3(& J23100): 200ul on ampicillin - pSB1K3: 200ul on kanamycin - pSB1A7: 50ul on ampicillin 50ul on kanamycin 50ul on no antibiotic - dH2O: 50ul on ampicillin 50ul on kanamycin 50ul on no antibiotic
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