840:153g:Projects/project27/2012/09/20
From OpenWetWare
< 840:153g:Projects | project27 | 2012 | 09(Difference between revisions)
(→Entry title) |
Current revision (19:01, 5 December 2012) (view source) (→Entry title) |
||
| (One intermediate revision not shown.) | |||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
| - | * Transformed chemically competent ''E. coli'' cells with | + | * Transformed chemically competent ''E. coli'' cells with J62001 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control) |
* After transformation, cultures were plated in the following quantities on antibiotic-containing media: | * After transformation, cultures were plated in the following quantities on antibiotic-containing media: | ||
| - | - | + | - J62001(& J23100): 200ul on ampicillin |
- pSB1K3: 200ul on kanamycin | - pSB1K3: 200ul on kanamycin | ||
Current revision
 Project name
|  Main project page Previous entry Next entry
|
Entry title
- J62001(& J23100): 200ul on ampicillin - pSB1K3: 200ul on kanamycin - pSB1A7: 50ul on ampicillin
50ul on kanamycin
50ul on no antibiotic
- dH2O: 50ul on ampicillin
50ul on kanamycin
50ul on no antibiotic
| |
