840:153g:Projects/project27/2012/09/20: Difference between revisions

Latest revision as of 22:02, 26 September 2017

Project name

Transformed chemically competent E. coli cells with J62001 plasmid carrying J23100 promoter, pSB1K3 linear plasmid backbone, pSB1A7 (+ control), and dH20 (- control)
After transformation, cultures were plated in the following quantities on antibiotic-containing media:

 - J62001(& J23100): 200ul on ampicillin

 - pSB1K3: 200ul on kanamycin

 - pSB1A7: 50ul on ampicillin
           50ul on kanamycin
           50ul on no antibiotic

 - dH2O: 50ul on ampicillin
         50ul on kanamycin
         50ul on no antibiotic

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+|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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