840:153g:Projects/project26/2012/11/29

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A Toxin</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Destination Plasmid Digestion==
==Destination Plasmid Digestion==
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* On Tuesday, November 27, we ran a gel on the plasmid digestion that was done on November 15.  The insert size is 1200 bp in length and the backbone is 4425 bp in length.  There were bands that appeared at the 1200 bp size and at the 3000+ bp size. The ladder was a 1000 bp ladder. From these results it is assumed that the digestion worked. This destination plasmid will be keep in storage for future classes to use.  
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* On Tuesday, November 27, we ran a gel on the plasmid digestion that was done on November 15.  The insert size is about 1200 bp in length and the backbone is 4425 bp in length.  There were bands that appeared at the 1200 bp size and at the 3000+ bp size. The ladder was a 1000 bp ladder. From these results it is assumed that the digestion worked. This destination plasmid will be keep in storage for future classes to use.  
[[Image:nov 27A.tif]]
[[Image:nov 27A.tif]]

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Cloning of Atrolysin A from Crotalus atrox Main project page
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Destination Plasmid Digestion

  • On Tuesday, November 27, we ran a gel on the plasmid digestion that was done on November 15. The insert size is about 1200 bp in length and the backbone is 4425 bp in length. There were bands that appeared at the 1200 bp size and at the 3000+ bp size. The ladder was a 1000 bp ladder. From these results it is assumed that the digestion worked. This destination plasmid will be keep in storage for future classes to use.

Image:nov 27A.tif


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