840:153g:Projects/project26/2012/08/30: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Cloning of Atrolysin A from ''Crotalus atrox''</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Locating Shed==
* Insert content here...
* After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone, it is the atrolysin A toxin. We were unable to find a DNA sequence but found an mRNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.




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__NOTOC__
__NOTOC__
[[category:OWWLabNotebookV1]]
[[category:OWWLabNotebookV1]]
After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone. Atrolysin A is the toxin. We were unable to find a dna sequence but found an RNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.

Revision as of 09:36, 4 December 2012

Cloning of Atrolysin A from Crotalus atrox <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Locating Shed

  • After contacting several zoos we tracked down a possible sample. We have found our toxin we wish to clone, it is the atrolysin A toxin. We were unable to find a DNA sequence but found an mRNA sequence. With this sequence we have to find the coding region and make primers that fit and work into the gene.