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Exp. 5 Digestion of Mini Prep and Exp. 6 Preparatory Digest & Purification
This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence. This gel picture shows our results:
Today (Thursday), we digested and purified part BBa_K20600 with Spe1 and Pst1 in order to prepare our part for ligation with our gene. We then purified the part using the GeneJET PCR Purification Kit and obtained two, 35μL samples. The first sample will be more concentrated than the second sample. Our future plans include running a gel to check the digestion and compare the intensity/quantity of our part and gene using Mass Ruler marker. The digestion will be comparable to Tuesday's (10/30)gel and the gene will be comparable to 10/11/2012 gel. They will be comparable by the cleanliness of the gel and the size of the bands.