840:153g:Projects/project25/2012/11/01

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This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence.  
This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence.  
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[[Image:2012-11-01 16.32.25.jpg|400px]]
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Revision as of 17:41, 1 November 2012

Cloning of mreB from Caulobacter crescentus into E. coli Main project page
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Exp. 5 Digestion of Mini Prep and Exp. 6 Preparatory Digest & Purification

This week we finished up experiment 5 by performing a digestion of the mini preps. By digesting our mini preps with EcoR1 and Pst1, we were able to separate our plasmid backbone from the promoter sequence.

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