840:153g:Projects/project25/2012/09/20: Difference between revisions

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Tango Alpha
Tango Alpha


    Bess Lippmann
Bess Lippmann
    Colby Swanson   
Colby Swanson   


Project Name and Description
Cloning of mreB from ''Caulobacter crescentus'' in ''E. coli''


    Explain your experimental design here
This week we were able to successfully culture C. crescentus and finish preparing reagents for DNA extraction. We loop inoculated 3 cultures in 5mL of PYE media which incubated at 30 degrees Celcius for 2 days. On 9/20/12 we began the DNA extraction using a protocol found on Open Wet Ware. We took two samples (A & B)to use for extraction and froze the remaining sample for future use. We did not finish the extraction due to time so froze our samples in the -80 degree freezer. Next week we plan on finishing this procedure as well as prepping for PCR to make sure our DNA is present. If time allows we will actually run the PCR that day.
    List all the steps that are needed to complete your project
    Do not go into experimental details and don't list procedures. Just list the major steps necessary to complete your project
    Please also make yourself familiar with uploading pictures and *.ppt files
 
Important Results and Milestones
 
    keep track of your most important results and refer to the corresponding page in your notebook
    upload important pictures (don't forget to label them! Powerpoint is very convenient). Remember: these will become quite handy later in your summary report or final presentation. If you do label and upload the pictures as soon as you got them, your summary report can be written much more effortlessly (do you usually procrastinate? This is chance to do some work before hand that frees you up for finals week).  
 
Recent changes

Revision as of 16:09, 20 September 2012

Tango Alpha

Bess Lippmann Colby Swanson

Cloning of mreB from Caulobacter crescentus in E. coli

This week we were able to successfully culture C. crescentus and finish preparing reagents for DNA extraction. We loop inoculated 3 cultures in 5mL of PYE media which incubated at 30 degrees Celcius for 2 days. On 9/20/12 we began the DNA extraction using a protocol found on Open Wet Ware. We took two samples (A & B)to use for extraction and froze the remaining sample for future use. We did not finish the extraction due to time so froze our samples in the -80 degree freezer. Next week we plan on finishing this procedure as well as prepping for PCR to make sure our DNA is present. If time allows we will actually run the PCR that day.

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