840:153g:Projects/project23/2012/11/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/11/29 Entry for 840:153g:Projects/project23)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
* Insert content here...
* On Tuesday this week, we prepared 12 tubes with 5 mL LB liquid media/ 5mcL kanamycin.
* On Wednesday, we innoculated 6 random colonies from Plate B (350bp fragment), and 6 random colonies from Plate F(450bp fragment), each in seperate tubes prepared Tuesday. We allowed these to shake at 200 RPM/ 37°C overnight.
* On Thursday, we isolated plasimd DNA from each of the tubes innoculated on Wednesday.  Glycerol stocks were then created from the remaining cultures for each.  We then digested each mini-prep with PstI and XbaI enzymes to release our inserted fragment.  These were allowed to run on a 0.8% agarose gel to determine whether the transformation from Nov. 15th was successful.




Latest revision as of 22:18, 26 September 2017

Project name Main project page
Previous entry      

Entry title

  • On Tuesday this week, we prepared 12 tubes with 5 mL LB liquid media/ 5mcL kanamycin.
  • On Wednesday, we innoculated 6 random colonies from Plate B (350bp fragment), and 6 random colonies from Plate F(450bp fragment), each in seperate tubes prepared Tuesday. We allowed these to shake at 200 RPM/ 37°C overnight.
  • On Thursday, we isolated plasimd DNA from each of the tubes innoculated on Wednesday. Glycerol stocks were then created from the remaining cultures for each. We then digested each mini-prep with PstI and XbaI enzymes to release our inserted fragment. These were allowed to run on a 0.8% agarose gel to determine whether the transformation from Nov. 15th was successful.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project23/2012/11/29&oldid=1011643"