840:153g:Projects/project23/2012/11/15: Difference between revisions
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#350bp 1x DNA | #350bp 1x DNA | ||
#100bp Ladder | #100bp Ladder | ||
[[image:Gel 11.13.12.jpg|600px]] | |||
*Result: Wells are shown No. 10 - 1 above. Plasmid DNA is intact, and our fragment DNAs are in correct position. Well 9 is overrepresented slightly, and all other sample combinations are comparable. The samples are useable for transformation, but in differing ratios to find the most effective combination. | |||
* We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0.5μL of plasmid DNA to our insert DNA (OmcF DNA). We also did 2 controls for each 450 and 350 DNA sample. One with no plasmid DNA and one with no insert DNA | * We then ligated the samples with concentrations of 4:4, 0.5:7.5, and 7.5:0.5μL of plasmid DNA to our insert DNA (OmcF DNA). We also did 2 controls for each 450 and 350 DNA sample. One with no plasmid DNA and one with no insert DNA | ||
* On Thursday, we continued to work on the transformation, by actually combining our ligated samples with 40μL of E.coli cells, icing, heat shocking, then icing the samples. We then added SOC and shook the samples for an hour at 37°C. | * On Thursday, we continued to work on the transformation, by actually combining our ligated samples with 40μL of E.coli cells, icing, heat shocking, then icing the samples. We then added SOC and shook the samples for an hour at 37°C. | ||
* We then plated our samples on solid media plates. | * We then plated our samples on Kanamycin solid media plates. | ||
Revision as of 12:18, 29 November 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Cloning E.coli with the OmcF gene
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