840:153g:Projects/project23/2012/11/15: Difference between revisions
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* On Thursday, we continued to work on the transformation, by actually combining our ligated samples with 40μL of E.coli cells, icing, heat shocking, then icing the samples. We then added SOC and shook the samples for an hour at 37°C. | * On Thursday, we continued to work on the transformation, by actually combining our ligated samples with 40μL of E.coli cells, icing, heat shocking, then icing the samples. We then added SOC and shook the samples for an hour at 37°C. | ||
* We then plated our samples on solid media plates. | * We then plated our samples on Kanamycin solid media plates. | ||
Revision as of 14:39, 15 November 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Cloning E.coli with the OmcF gene
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