840:153g:Projects/project23/2012/11/02: Difference between revisions
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Casey Durnan (talk | contribs) (Autocreate 2012/11/02 Entry for 840:153g:Projects/project23) |
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==Entry title== | ==Entry title== | ||
* | * Today, we photographed the gel from 11.01.2012. After photographing both gels, we cut out the bands from the 350bp and 450bp regions. We stored these gel slices at -20°C for further DNA extraction next week. | ||
*1 percent agarose gel | |||
*1. 100-base pair ladder | |||
*2-4. Replicates of primered set | |||
[[image:11.09.12_gel_1.0.jpg]] | |||
*Conclusion: These samples banded like all others before. We can use this for further extraction. | |||
*1.8 percent agarose gel | |||
##Positive control | |||
##100 base-pair ladder | |||
##sfmr frag w/o DNA (neg control) | |||
##primered set w/o DNA (neg control) | |||
##mfsr frag w/o DNA (neg control) | |||
##100 base-pair ladder | |||
##sfmr frag w/ DNA | |||
##primered set w/ DNA | |||
##mfsr frag w/ DNA | |||
##100 base-pair ladder | |||
##primered replicate | |||
##primered replicate | |||
[[image:11.09.12_gel_1.8.jpg|600px]] | |||
*Conclusion: Positive and negative controls check ok. For comparison, the primered set does have new, distinct banding from both fragments. After further calculations, our total product length should be ~350bp. A previous calculation did not take the overlap region between fragments into consideration. We will extract both the 350 and 450-bp regions to be certain. The replicates also look usable in this gel. | |||
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__NOTOC__ | __NOTOC__ | ||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] |
Revision as of 19:48, 8 November 2012
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