840:153g:Projects/project22/2012/10/04

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(PCR Results and Retry)
(PCR Results and Retry)
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==PCR Results and Retry==
==PCR Results and Retry==
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On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.
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On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.
Wells 1-12 were ours (markers at 5 and 12 going right to left)
Wells 1-12 were ours (markers at 5 and 12 going right to left)

Revision as of 18:30, 4 October 2012

Prodigiosin Production by E. Coli. Main project page
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PCR Results and Retry

On Tuesday we ran simple gel electrophoresis of our PCR products. Unfortunately, our DNA's signal was very weak and the positive control failed as well. Today we attempted to concentrate the DNA in our samples in a Speed Vacuum Concentrator and will run PCR on this DNA on Tuesday to check said concentration. If the DNA is sufficiently concentrated, we will take another shot at PCR next week. We will first retest the positive control with a few of our primers to make sure the controls work properly before we go all in.

Wells 1-12 were ours (markers at 5 and 12 going right to left) [1]

Wells 18-20 were ours (negative controls and marker, far left side) [2]

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