840:153g:Projects/project20/2011/10/13

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electrophoresis for pcr production and transformation agian for our promotors

  • after the gel, we found that the most briht bands' BP were less than waht we expected.
  • in the posion where our expected band should be, we had weak band. so, we need pcr again by using diff tm to see if we can get more in that posiotion.
  • transform 3 promoters into e.coli, see if we can get a useful promoter for recombine( forgot +control this time)
  Part:BBa_K091112   Part:BBa_K206001   Part:BBa_I0500
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