840:153g:Projects/project2/2008/09/16: Difference between revisions
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==The Sweet Smell of.....''E.coli''?== | ==The Sweet Smell of.....''E.coli''?== | ||
Updated Proposal with how we will test our third and fourth questions, optimal tyrosine in media and amount of time from red light to vanillin production respectively. (Aaron Miller) | |||
Updated proposal | Updated proposal | ||
Revision as of 11:09, 16 September 2008
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The Sweet Smell of.....E.coli?Updated Proposal with how we will test our third and fourth questions, optimal tyrosine in media and amount of time from red light to vanillin production respectively. (Aaron Miller) Updated proposal Red light sensitive E.coli that produces vanillin
The system that we wish to put together will be made up of two devices and one part from the iGEM registry:
2.Device BBa_M30109: Involved in light sensitivity
3.Part BBa_R0082: The OmpF promoter region
In addition to the devices and part listed above, the following parts will also be utilized in the project:
One component of our project which is not available to us through iGEM registry:
The proposed order of arrangement of the chosen devices and parts:
[All biobricks in the iGEM library can be combined using restriction digestion and ligation method.] The basic steps involved in our project: 1) Make competent cells (DH5 alpha) that would house the vectors containing the extracted devices and parts from the iGEM registry. The vector that will be used to clone the biobricks is pBlueScript. 2) Obtain the parts and devices from the iGEM registry, store those parts in E. coli colonies.
3) Restriction digestion and ligation of devices and part in the order described above, verification using acylamide gel electrophoresis. 4) Ligate system into vector pTG 262 (BBa_I1742123), transform EnvZ deficient E.coli 5) Selection of positive colonies (colonies without red fluorescence and resistant to Chloramphenicol) and test system functionality (presence of vanilla smell) We will attempt to answer questions related to characterization of the system:
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