840:153g:Projects/project16/2011/09/29

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PCR

Today we set up a PCR for our two DNA samples; the phenol/chloroform extraction and the phenol/chloroform coupled with ethanol precipitation. The negative control consisted of only primers in the PCR mix and the positive controls were plasmid DNA with plasmid primers and plasmid primers by themselves. We chose the temperatures of 43 C, 48 C and 52.1 C The results of this will determine if our method for DNA extraction will be effective to proceed in our project.


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