840:153g:Projects/project13/2010/10/28: Difference between revisions

Revision as of 14:22, 28 October 2010

We digested part BBa_J23119 with enzymes spel and pstl to cut this part open and leaving the plasmid attached attached to the "E" prefix.
We also purified part BBa_J23119 to get rid of the left over base pairs.
We ligated our part BBa_J23119 with our oligo, thus making a promoter and an ERE part attached to a vector.
We transformed this ligated part (BBa_J23119 and the oligos)into competenet cells and plated them.

We received our ERE part (BBa_K123002) it is the same gene sequence as our oligos, but the oligos has the sticky ends we needed.
We plated this part, and collected colonies.
We purified the DNA and stored the DNA for possible future use.

We will count and collect colonies tomorrow for our ligated part(BBa_J23119 and our oligos).
We plan to run a electrophorysis gel to check our ligated part (BBa_J23119 and our oligos)has been taken up properly by the competent cells.

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-We have found all the parts we need on BioBricks (BBa_K123003, BBa_K123002, BBa_E0240, and BBa_E0840)
+* We digested part BBa_J23119 with enzymes spel and pstl to cut this part open and leaving the plasmid attached attached to the "E" prefix.
-We found all the parts in the 2010 Biobricks kit and then resuspended the DNA
+* We also purified part BBa_J23119 to get rid of the left over base pairs.
-We also poured our ampicillian plates
+* We ligated our part BBa_J23119 with our oligo, thus making a promoter and an ERE part attached to a vector.
+* We transformed this ligated part (BBa_J23119 and the oligos)into competenet cells and plated them.
+* We received our ERE part (BBa_K123002) it is the same gene sequence as our oligos, but the oligos has the sticky ends we needed.
+* We plated this part, and collected colonies.
+* We purified the DNA and stored the DNA for possible future use.
+* We will count and collect colonies tomorrow for our ligated part(BBa_J23119 and our oligos).
+* We plan to run a electrophorysis gel to check our ligated part (BBa_J23119 and our oligos)has been taken up properly by the competent cells.