840:153g:Projects/project13/2010/10/14: Difference between revisions

Latest revision as of 14:48, 14 October 2010

We have now selected our promoters/RFP's ( red flourescent protein). Our promoter is BBa_J23119. The 2 RFP's with plasmid that allows future splicing between promoter and RFP are BBa_J23102 and BBa_J23100. Our last BioBrick that we selected contains a promoter, RBS, RFP, and terminator, BBa_J04450.
we then transformed all of these parts into competent cells and grew them on agar plates.
On Wednesday we checked colony growth and found that we had growth on all but one, BBa_J23102 (using only 2μL.) We numbered and picked clonies to grow in LB medium.
Today we worked on our plasmid DNA prepartion and storage of all four of these parts.
Next week we plan to digest our plasmid DNA and run an electrophoresis gel. We will also resuspend our oligos DNA.

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-We have found all the parts we need on BioBricks (BBa_K123003, BBa_K123002, BBa_E0240, and BBa_E0840)
+* We have now selected our promoters/RFP's ( red flourescent protein). Our promoter is BBa_J23119. The 2 RFP's with plasmid that allows future splicing between promoter and RFP are BBa_J23102 and BBa_J23100.  Our last BioBrick that we selected contains a promoter, RBS, RFP, and terminator, BBa_J04450.
-We found all the parts in the 2010 Biobricks kit and then resuspended the DNA
+* we then transformed all of these parts into competent cells and grew them on agar plates.
-We also poured our ampicillian plates
+* On Wednesday we checked colony growth and found that we had growth on all but one, BBa_J23102 (using only 2μL.) We numbered and picked clonies to grow in LB medium.
+*  Today we worked on our plasmid DNA prepartion and storage of all four of these parts.
+* Next week we plan to digest our plasmid DNA and run an electrophoresis gel.  We will also resuspend our oligos DNA.