840:153g:Projects/project11/2010/10/28

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Tuesday we digested our Promoter(BBa_I2007) with Spe1. It was then purified using the GeneJET PCR purification kit. Ligated Promoter with the RBS that we created from oligos. We also ligated the gene(BBa_E1010) to the double terminator (BBa_B0015). Store in refrigerator overnight.

Thursday we transformed E.coli with the ligated parts. Plated the trasnformed bacteria onto antibiotic plates. We plan to incubate overnight, then store in refrigerator until Monday when we will grow liquid cultures. During next week, we will isolate plasmids, cut and see if parts were ligated correctly by running on a gel.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project11/2010/10/28&oldid=468686"