840:153g:Projects/project10/2010/11/04: Difference between revisions
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Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. It did, bands were faint but showed up around 900 bp mark. Top well is | Decided to insert DNA into T-plasmid vector. Purified our PCR product using QIAquick kit. Ran another gel to see if purification succeeded. It did, bands were faint but showed up around 900 bp mark. Top well is S&C primers, middle is ladder, bottom is G&C primers. | ||
[[Image:Purification gel.jpg]] | [[Image:Purification gel.jpg]] | ||
Revision as of 14:52, 4 November 2010
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleDiscovered that once again RNA extraction failed.Gel showed no trace of cDNA as all samples were the same length, both controls and samples from RT-PCR. First two lanes are G & C primers, middle is ladder, and last two lanes are S &C primers.
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