We received our primers Cowboy, Cowgirl, Sneezy, Grumpy. Set up and ran PCR's with all combinations of forward and reverse primers at 3 different temperatures (48C, 51.7C, and 58C) because of differences in annealing temperatures - chose the lowest and went 5 degrees below it and 5 degrees above it. Next, we took the PCR products and set up an agarose gel electrophoresis to determine if the primers work and if certain pairing(s) work better than others. Ran gel at 200Volts for 40 minutes. Will see results next week. Next week, we will begin RNA extraction.
Our bands are shown on the bottom. PCR was successful, primers are working, and there is amplification of DNA.