840:153g:Projects/project10/2010/10/07: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Gabe Martin (talk | contribs) (Autocreate 2010/10/07 Entry for 840:153g:Projects/project10) |
Gabe Martin (talk | contribs) No edit summary |
||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
* | * We received our primers Cowboy, Cowgirl, Sneezy, Grumpy. Set up and ran PCR's with all combinations of forward and reverse primers at 3 different temperatures (48C, 51.7C, and 58C) because of differences in annealing temperatures - chose the lowest and went 5 degrees below it and 5 degrees above it. Next, we took the PCR products and set up an agarose gel electrophoresis to determine if the primers work and if certain pairing(s) work better than others. Ran gel at 200Volts for 40 minutes. Will see results next week. Next week, we will begin RNA extraction. | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 14:19, 7 October 2010
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry title
| |
