840:153g:Projects

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840:153g: Recombinant DNA Te(a)chniques

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We have 16 students in class which will form groups of two throughout the semester. Each group will clone their own gene of interest into E. coli and verify that they cloned a functional gene. Students have to select a gene and identify a suitable source for cloning it. They need to develop a strategy for cloning, including a procedure for verification that they actually cloned the full coding region of the gene. Each group of students will develop a website at OWW where they describe their project. It is required that student write a DAILY lab notebook during/after each lab session. This online notebook must contain all procedures carried out during the session, description of results, and discussion of the next steps. This notebook will be graded based on completeness, accuracy, and content. It will substitute for a paper lab notebook.