7-AAD

7-aminoactinomycin-D

Purpose

• Staining dead cells

Procurement

• Most standard companies
  • BD Biosciences
  • eBioscience

Use

• 546/647
• Nucleic acid dye
• Often used in conjunction with Annexin-V when assaying for apoptotic cells
• "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

• No significant safety considerations (BD Biosciences MSDS Sheet)

References

• MC O’Brien, et al. (1995) Cytometry 19:243
• I Schmid et al. (1992) Cytometry 13:204