7-AAD

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7-aminoactinomycin-D
7-aminoactinomycin-D
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==Purpose==
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* Staining '''dead cells'''
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==Procurement==
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* Most standard companies
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** [http://www.bdbiosciences.com/ptProduct.jsp?prodId=12856 BD Biosciences]
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** [http://www.ebioscience.com/ebioscience/specs/antibody_00/00-6993.htm eBioscience]
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==Use==
* 546/647
* 546/647
* Nucleic acid dye
* Nucleic acid dye
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* Stains '''dead cells'''
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* "The '''advantage of 7-AAD''' over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
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* "The advantage of 7-AAD over PI is the ability to be used '''in conjunction with''' phycoerythrin ('''PE''')- '''and''' fluorescein isothiocyanate ('''FITC''')-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the '''far red range''' of the spectrum (650 nm long-pass filter).
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==Safety==
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* No significant safety considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/559925-MSDS.01.pdf BD Biosciences MSDS Sheet])
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==References==
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* MC O’Brien, et al. (1995) Cytometry 19:243
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* I Schmid et al. (1992) Cytometry 13:204
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Revision as of 03:47, 8 July 2008

7-aminoactinomycin-D

Contents

Purpose

  • Staining dead cells

Procurement

Use

  • 546/647
  • Nucleic acid dye
  • "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

References

  • MC O’Brien, et al. (1995) Cytometry 19:243
  • I Schmid et al. (1992) Cytometry 13:204
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