7-AAD: Difference between revisions
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Latest revision as of 06:30, 5 May 2009
7-aminoactinomycin-D
Purpose
- Staining dead cells
Procurement
- Most standard companies
Use
- 546/647
- Nucleic acid dye
- Often used in conjunction with Annexin-V when assaying for apoptotic cells
- "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Safety
- No significant safety considerations (BD Biosciences MSDS Sheet)
References
- MC O’Brien, et al. (1995) Cytometry 19:243
- I Schmid et al. (1992) Cytometry 13:204
