3-Aminotriazole - Revision history

Torsten Waldminghaus at 08:54, 7 March 2008

Torsten Waldminghaus — Fri, 07 Mar 2008 08:54:27 GMT

←Older revision		Revision as of 08:54, 7 March 2008
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	==Procurement==		==Procurement==
-	You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].	+	*You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].

	==Use==		==Use==
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	==References==		==References==
-	[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]	+	*[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Torsten Waldminghaus: adapted to template

Torsten Waldminghaus — Fri, 07 Mar 2008 08:53:31 GMT

adapted to template

←Older revision		Revision as of 08:53, 7 March 2008
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-	3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.	+	==Purpose==
		+	*3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

-	~~Stored as a powder at~~ -~~20. Working concentrations vary depending on the desired stringency~~, ~~from 0.~~2 ~~mM to 25mM~~.	+	==Procurement==
		+	You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].

-	~~Possible carcinogen.~~ Use ~~gloves, work in~~ a ~~hood~~.	+	==Use==
		+	*Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

-	References:	+	==Safety==
		+	*Possible carcinogen. Use gloves, work in a hood.
		+
		+	==References==
	[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]		[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Reshma P. Shetty at 17:30, 19 October 2007

Reshma P. Shetty — Fri, 19 Oct 2007 17:30:32 GMT

←Older revision		Revision as of 17:30, 19 October 2007
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	References:		References:
	[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]		[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
		+
		+	[[Category:Material]] [[Category:Chemical]]

Josh K. Michener at 21:07, 31 July 2006

Josh K. Michener — Mon, 31 Jul 2006 21:07:11 GMT

New page

3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Possible carcinogen. Use gloves, work in a hood.

References:
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]