3-Aminotriazole

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Current revision (04:54, 7 March 2008) (view source)
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==Procurement==
==Procurement==
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You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
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*You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
==Use==
==Use==
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==References==
==References==
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[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
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*[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
[[Category:Material]] [[Category:Chemical]]
[[Category:Material]] [[Category:Chemical]]

Current revision

Contents

Purpose

  • 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Procurement

  • You can buy 3-amino-1,2,4-triazole for example at Sigma.

Use

  • Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Safety

  • Possible carcinogen. Use gloves, work in a hood.

References

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