3-Aminotriazole
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==Procurement== | ==Procurement== | ||
| - | You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma]. | + | *You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma]. |
==Use== | ==Use== | ||
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==References== | ==References== | ||
| - | [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] | + | *[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information] |
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Current revision
Contents |
Purpose
- 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
Procurement
- You can buy 3-amino-1,2,4-triazole for example at Sigma.
Use
- Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
Safety
- Possible carcinogen. Use gloves, work in a hood.
