3-Aminotriazole

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3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
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==Purpose==
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*3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
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Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
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==Procurement==
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You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
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Possible carcinogen. Use gloves, work in a hood.
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==Use==
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*Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
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References:
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==Safety==
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*Possible carcinogen. Use gloves, work in a hood.
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==References==
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
[[Category:Material]] [[Category:Chemical]]
[[Category:Material]] [[Category:Chemical]]

Revision as of 03:53, 7 March 2008

Contents

Purpose

  • 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Procurement

You can buy 3-amino-1,2,4-triazole for example at Sigma.

Use

  • Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Safety

  • Possible carcinogen. Use gloves, work in a hood.

References

Sigma product information

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