2020(S11) Lecture:week 1

Week 1 Tuesday

GOT DNA?

After a short introduction to the who/what/why for this class, we'll start handling the programming material that's at our fingertips...in our fingertips, even! As you work through this DNA isolation protocol below, be mindful of your questions and your own reactions to this activity. Is this the first time you've ever handled a lump of DNA? How hard is it to isolate DNA, both technically speaking and in terms of the needed equipment? What surprised you? What did you learn and what questions do you still have? What could you do next with this material you've isolated?

DNA from a Strawberry

1. Pair up and pick up a ziplock baggie with a strawberry in it. IF YOU ARE ALLERGIC TO STRAWBERRIES, please don't do this part.
2. Push the air out of the baggie as best you can and reseal it.
3. Being careful not to break the baggie, mash the strawberry with your fingers for ~2 minutes.
4. Using a medicine cup to measure it, add 10 ml of extraction buffer to the baggie. Extraction buffer is a mixture of 20 ml dish soap, 1.5 tsp of table salt, a sprinkle of meat tenderizer, and 380 ml of spring water.
5. Mash the strawberry for one minute more.
6. Place a funnel over a 3oz. bathroom cup.
7. Fold a piece of cheesecloth into a ~4 inch square and place it in the funnel.
8. Pour the strawberry mush into the funnel and allow gravity to do its work. You'll throw away the strawberry mush and cheesecloth into the trash, and return the funnel to the kit.
9. Carefully pour 2 ml of the filtered strawberry juice into a clean, conical tube, using the markings on the side of the tube to know how much to transfer.
10. Hold the tube at an angle and, using an eye-dropper, carefully DRIBBLE a 5 ml layer of cold 95% Ethanol or 70% isopropanol (your choice) onto the strawberry goop. DO NOT MIX the layers.
11. Now watch...after a few minutes you should start to see some bubbles, then some white stringy stuff, which is the DNA.
12. There will be coffee stirrers to remove the DNA from the tube if you'd like to do that. The DNA is best removed by "spooling"--i.e. winding the DNA around the stick with a gentle twirl of stick.

Why are we doing this?? Let's return to the questions from the start of the day: Was this the first time you've ever handled a lump of DNA? Is it what you thought it would look like? Is there more or less than you thought there'd be? How do you know it's DNA? How hard was it to isolate DNA, technically speaking? needed equipment? What surprised you? What did you learn and what questions do you now have? Where did you finally put the DNA you isolated? What did you do with it after you isolated it? Is it OK to just throw it away?

Week 1 Studio

Wednesday matinee

Instructions: Today you will have the opportunity to watch a video showcasing completed iGEM projects. "iGEM" stands for the "international Genetically Engineered Machines" competition. It is a summer-long opportunity for teams of students working at colleges and universities around the world to design and build genetically engineered machines, many of which use standard biological parts from the Registry of Standard Biological Parts. The video will orient you to the kinds of accomplishments realized in a summer by teams of undergraduates and their advisers.

Our feature presentation

Eau D'coli

Our featured presentation will emphasize some of the "engineering" that can be accomplished in a summer by a talented group of undergrads much like yourselves. We'll listen to

• the 2006 iGEM team from MIT describing a neat project for fine chemical synthesis presentation video presentation ppt
  

Their project will allow us to focus on

• • programming genetic logic, growth phases of bacterial cultures, ethical questions of human experimentation
    • our ppt review of these processes is here
      
      

After the presentation

You will have 10 minutes to gather with your fellow moviegoers and discuss what you saw, using these "iGEM review questions" as a guide for your conversations:

1. what was the problem this team chose to address and why?
2. is this an important problem and why or why not?
3. did they succeed in part or in total?
4. are there aspects of the work that are unclear to you?
5. if you could ask this team one question what would it be?

Our second feature

There have been many wonderful projects from teams over the years, but to narrow down the number of possible choices, let's look at the projects from recent MIT teams. As a class we'll choose one of the following videos to watch and discuss:

• MIT 2008: Biogurt, fighting tooth decay since 2008,presentation video
• MIT 2009: Phycocyanobilibuddies, photolocalizer project,presentation video
• MIT 2010:Programmable, self-constructing biomaterials, presentation video

After the presentation

Take time again with your fellow movie goers to discuss what you saw

1. what was the problem this team chose to address and why?
2. is this an important problem and why or why not?
3. did they succeed in part or in total?
4. are there aspects of the work that are unclear to you?
5. if you could ask this team one question what would it be?

After the show

Before you leave today, consider the topic areas that are prized by iGEM judges. In 2010, prize areas were:

Area	Challenge
Food or Energy	People need to eat. Planes, trains, and automobiles need to eat too. Can biotechnology be responsibly used to produce food or energy without causing widespread shortages of either, and without harming the environment that future generations will inherit?
Environment	The quality of the air, water, and land, both on Earth and other heavenly bodies, limits the happiness of humans and other creatures. Can biotechnology be used to help clean the air, provide fresh drinking water, restore or enhance soil quality, terraform a near-Earth asteroid, or protect, preserve, or enhance natural biological diversity?
Health or Medicine	Many health and medical problems might best be addressed by improved biological technologies. What can synthetic biology do?
Manufacturing	Have you ever heard of nanotechnology? Well, biology is a nanotechnology that already exists, and that actually works. The ribosome is a programmable nanoassembler embedded within a reproducing machine. Forget grey goo, we've got green goo, and it has already taken over the planet! Thus, could we responsibly use biology to manufacture useful products, from the nanoscale (atoms) to the decascale (buildings and bridges). What can biology be programmed to manufacture?
New Application Area	We're guessing that you have great ideas that nobody has ever thought about, or if they have they forgot to tell somebody else. Can you imagine an entirely new application area for biological technology? Go for it. We'll celebrate you in front of the entire world
Foundational Advance	Modern biotechnology dates back to the invention of recombinant DNA technology, which lets people cut and paste pre-existing fragments of genetic material. That was only 35 years ago. In other words, biotechnology is a young adult, just entering its prime years. One thing that desperately needs doing is to develop improved tools and technologies that help to make the entire process of engineering biology easier. What foundational advance can you contribute that will take everybody's work forward?
Information Processing

BEFORE YOU LEAVE TODAY

Put your name on a piece of paper or index card and list your top 3 areas of interest. Hand in this list before you leave.
This term you'll be on a team of 20.020 students and work with 20.385 mentors to design and specify an iGEM-like system in one of these areas. You won't get to build it unless you sign on for the iGEM summer, but many 20.020 students have gone on to do just that...

HOMEWORK Next time we will work with the banana-smell generating system. In advance of this activity, please review the set up of the challenge by looking at the BioBuilder BioPrimer #1. If you need a refresher on some of the terminology (inverter?? stationary phase promoter??), then you can watch the animations that are linked to the BioPrimer from the BioBuilder website.

Week 1 Thursday

Why are we doing this?