20.109:Module 4: Difference between revisions
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==Module 4== | ==Module 4== | ||
'''Instructors:''' Angela Belcher and [[Natalie Kuldell]] | '''Instructors:''' [[Angela Belcher]] and [[Natalie Kuldell]] | ||
'''TA:''' [[Chandni Valiathan]] | '''TA:''' [[Chandni Valiathan]] | ||
In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we’ll elucidate some amino acid requirements for the yeast/metal interaction. | |||
[[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']] | |||
[[20.109(S07):Bio-material engineering/Screening library | Module 4 Day 1: Screening library]]<br> | |||
[[20.109(S07): Bio-material engineering/Optimizing panning | Module 4 Day 2: Optimizing panning]]<br> | |||
[[20.109(S07): Bio-material engineering/Rescreening gold binders | Module 4 Day 3: Rescreening gold binders]]<br> | |||
[[20.109(S07): Bio-material engineering/PCR of gold binding candidates | Module 4 Day 4: PCR of gold binding candidates]]<br> | |||
[[20.109 (S07): Research proposal, module 4| Module 4 Day 5: Student presentations]]<br> | |||
[[20.109(S07): Bio-material engineering/Sequence analysis | Module 4 Day 6: Sequence analysis]]<br> | |||
[[20.109(S07): Biomaterial engineering, TA notes| TA notes Module 4]] | |||
Latest revision as of 03:30, 17 April 2007
Module 4
Instructors: Angela Belcher and Natalie Kuldell
In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we’ll elucidate some amino acid requirements for the yeast/metal interaction.
Module 4 Day 1: Screening library
Module 4 Day 2: Optimizing panning
Module 4 Day 3: Rescreening gold binders
Module 4 Day 4: PCR of gold binding candidates
Module 4 Day 5: Student presentations
Module 4 Day 6: Sequence analysis
TA notes Module 4
