20.109(S14): TA notes for module 2

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(Day 2)
(General notes)
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==General notes==
==General notes==
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<font color=red>These TA notes need to be written… in progress. I also need to figure out the quiz dates.</font color>
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<font color=red> I need to figure out the quiz dates.</font color>
See also GoogleDoc for aliquoting amounts.
See also GoogleDoc for aliquoting amounts.
'''Scheme:'''  
'''Scheme:'''  
 +
-Plate wild type K1 and Ku80 deficient xrs6 cells
 +
-Collect lysates for Westerns to measure Ku80
 +
-Prepare damaged DNA plasmids of varying topologies
 +
-Transfect K1 and xrs6 cells with damaged DNA
 +
-Measure DNA damage repair via NHEJ by flow cytometry
'''Key preparation:'''
'''Key preparation:'''
 +
Note to TA:
* While you read the wiki, test each link, and make any link descriptions bold that are not already.
* While you read the wiki, test each link, and make any link descriptions bold that are not already.
* As you read the protocols, add reagent calculations to the GoogleDoc as needed.
* As you read the protocols, add reagent calculations to the GoogleDoc as needed.

Revision as of 00:54, 12 March 2014

20.109(S14): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2014        Assignments       
Module 1        Module 2        Module 3              

Contents

General notes

I need to figure out the quiz dates.

See also GoogleDoc for aliquoting amounts.

Scheme: -Plate wild type K1 and Ku80 deficient xrs6 cells -Collect lysates for Westerns to measure Ku80 -Prepare damaged DNA plasmids of varying topologies -Transfect K1 and xrs6 cells with damaged DNA -Measure DNA damage repair via NHEJ by flow cytometry

Key preparation:

Note to TA:

  • While you read the wiki, test each link, and make any link descriptions bold that are not already.
  • As you read the protocols, add reagent calculations to the GoogleDoc as needed.

Day-by-day

Day 1

Materials required: None


Day of Lab (T/W):


After Lab:


How it went:

Day 2

Materials required: Please read as much as you can of Parts 1-3 and create an automated worksheet (e.g., in Excel) that will perform the required calculations for the day. A partially prepared worksheet is linked here. We will NOT collect your final worksheet this time; it is only for your benefit.

Day of Lab (R/F):

How it went:

Day 3

Materials required:


Day of Lab (T/W):

After Lab:

How it went:

Day 4

Materials required:

Day of Lab (R/F):

After Lab:

How it went:

Day 5

Materials required:

Day of Lab (T/W):

After Lab:

How it went:

Day 6

Materials required:

Day of Lab (R/F):

After Lab:

How it went:

Day 7

Materials required:

Day of Lab (T/W):

After Lab:

How it went:

Day 8

Materials required:

Day of Lab (R/F):

After Lab:

How it went:
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