20.109(S13): TA notes for module 1

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(New page: {{Template:20.109(S13)}} <div style="padding: 10px; width: 640px; border: 5px solid #99FF66;"> Coming soon! ==General notes== '''Scheme:''' '''Key preparation:''' ===Day 1=== '''Ma...)
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==General notes==
==General notes==
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See also GoogleDoc for aliquoting amounts.
'''Scheme:'''  
'''Scheme:'''  
'''Key preparation:'''
'''Key preparation:'''
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 +
==Day-by-day==
===Day 1===
===Day 1===
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'''Day of Lab (R/F):'''
'''Day of Lab (R/F):'''
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 +
'''After Lab:'''
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'''How it went:''' Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.
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===Day 2===
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'''Materials required:'''
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*Bird samples! At 100 &mu;L each in a '''2 mL''' tube
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*Aliquots of components from QiaAMP stool kit (see GoogleDoc)
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*InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one
 +
 +
'''Day of Lab (R/F):'''
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*Check ASL and AL for precipitates; pre-heat at about 40-50 &deg;C in the water bath at the TA bench
 +
*Set up the heat block at the front bench. Fill tube slots with water and set at 70 &deg;C
 +
'''After Lab:'''
'''After Lab:'''
'''How it went:'''
'''How it went:'''

Revision as of 09:37, 10 February 2013

20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
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Coming soon!

Contents

General notes

See also GoogleDoc for aliquoting amounts.

Scheme:

Key preparation:

Day-by-day

Day 1

Materials required:

Day of Lab (R/F):

After Lab:

How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.

Day 2

Materials required:

  • Bird samples! At 100 μL each in a 2 mL tube
  • Aliquots of components from QiaAMP stool kit (see GoogleDoc)
  • InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one

Day of Lab (R/F):

  • Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
  • Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C


After Lab:

How it went:
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