20.109(S13): Primer design summary

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(Logistics)
(Logistics)
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==Logistics==
==Logistics==
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Please email your completed summary to <font color = 990000>'''20109 DOT submit AT gmail DOT com'''</font color>, with filename  <font color = 990000>'''Firstinitial_Lastname_LabSection_Mod1.doc'''</font color> (for example, S_Hockfield_TR_Mod2.doc).
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Please email your completed summary to <font color = 990000>'''20109 DOT submit AT gmail DOT com'''</font color>, with filename  <font color = 990000>'''Firstinitial_Lastname_LabSection_Mod1.doc'''</font color> (for example, R_Reif_TR_Mod2.doc).
This assignment is '''due by 11 am''' on March 19th (Tuesday) or March 10th (Wednesday), according to which day you have lab.
This assignment is '''due by 11 am''' on March 19th (Tuesday) or March 10th (Wednesday), according to which day you have lab.

Revision as of 20:45, 13 February 2013

20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Contents

Overview

You will write a brief memo about your study of diagnostic primers for identifying microsporidia.

The target audience is an advisor for whom you are preparing a relatively informal (but accurate!) report.

Logistics

Please email your completed summary to 20109 DOT submit AT gmail DOT com, with filename Firstinitial_Lastname_LabSection_Mod1.doc (for example, R_Reif_TR_Mod2.doc).

This assignment is due by 11 am on March 19th (Tuesday) or March 10th (Wednesday), according to which day you have lab.

Content Guidelines

Two primary sections -- one on design and one on results -- will make up the memo.

Design

Here you should include the following:

  • A short (1-2 paragraph) introduction motivating your design challenge and explaining your design choices.
  • A table comparing the properties of the V1-PMP2 primer set and your primer set.

The text related to your specific design choices should explicitly refer to the table.

Results

Here you should include the following:

  • A short (1-2 paragraph) description of your findings.
  • A figure depicting your raw PCR results (as seen on a gel).
  • Optionally, a figure or table depicting the PCR results in some processed form.
The figure should include a complete caption as for a publication.
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