20.109(S13):DNA sequencing and primer analysis (Day5)

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20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Contents

Introduction

miniprep

sequencing

Protocols

Part 1: Extract DNA from selected clones (mini prep)

deciding whether to do Qiagen or homebrew kit

Part 2: Measure DNA concentration

Part 3: Prepare sequencing reactions

Part 4: Count colonies

Part 5: Sensitivity/specificity analysis for microsporidia primers

For next time

Reagent list

write something here or not accessible to edit
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