20.109(S13):DNA cloning (Day4)

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Revision as of 17:05, 6 December 2012

20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Contents

Introduction

background on this cloning system -- vector arms and strain

transformation

Protocols

Part 1: Purify 16S PCR band from gel

Part 2: Ligate purified product to vector arms

Part 3: Transform ligated product into cloning strain

For next time

Reagent list

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