20.109(S13):DNA cloning (Day4)

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(Introduction)
(Protocols)
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==Protocols==
==Protocols==
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===Part 1: Purify 16S PCR band from gel===
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===Part 2: Ligate purified product to vector arms===
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===Part 3: Transform ligated product into cloning strain===
==For next time==
==For next time==
==Reagent list==
==Reagent list==

Revision as of 13:20, 6 December 2012

20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Contents

Introduction

background on this cloning system -- vector arms and strain

transformation

Protocols

Part 1: Purify 16S PCR band from gel

Part 2: Ligate purified product to vector arms

Part 3: Transform ligated product into cloning strain

For next time

==Reagent list==
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