20.109(S13):Context-setting and primer design (Day1)

From OpenWetWare

Revision as of 15:38, 13 December 2012 by AgiStachowiak (Talk | contribs)
Jump to: navigation, search
20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              

Contents

Introduction

  • overall motivation

Identifying pathogens in humans can suggest appropriate course of treatment. Identifying pathogens in other species can be both intellectually interesting and have applications for human health

Molecular identification of pathogens as powerful and perhaps less subjective than the identification according to morphology and phenotype

Requires a highly conserved gene. Something essential and little tolerance of mutation. Hence rRNA.

[Clarridge review]

  • background about small subunit rRNA


  • bacteria-specific background
  • microsporidia-specific background

Over 1000 species, with 14 infecting humans

Not a time of full genome sequence information

[Broad initiative]

Cites for bird infections and zoonotic potential

Parallels to avian flu

  • primer design basic background here or directly in protocols?

Protocols

Part 1: Lab practical

You and your partner may work together on the lab practical. (Note: this will not be the case for future quizzes.) You are of course welcome to give different answers should you disagree.

Part 2: Explore existing microsporidia-specific primers

Part 3: Design novel microsporidia-specific primers

For next time

Reagent list

write something here or not accessible to edit
Personal tools