20.109(S09)/PinkWF

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(Project Overview)
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Our goal is to rewire a bacterium to "miniprep" itself, when given some sort of an external stimuli (such as sensing of dense cell growth). This project will serve the following purposes:
Our goal is to rewire a bacterium to "miniprep" itself, when given some sort of an external stimuli (such as sensing of dense cell growth). This project will serve the following purposes:
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1) Make the research process easier, cheaper, and more efficient for scientists working with bacteria.
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* Make the research process easier, cheaper, and more efficient for scientists working with bacteria.
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2) Most laboratory strains have been genetically modified for scientific use; however our bacteria would be the first to be modified to do an experiment on itself.
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* Most laboratory strains have been genetically modified for scientific use; however our bacteria would be the first to be modified to do an experiment on itself.
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3) Self-lysing of cells when dense cell growth is encountered - less chance for environmental contamination if genetically engineered bacteria escape from the lab
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* Self-lysing of cells when dense cell growth is encountered - less chance for environmental contamination if genetically engineered bacteria escape from the lab
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2) Serve as an example of a way synthetic biology can be used for cellular control
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* Serve as an example of a way synthetic biology can be used for cellular control
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3) Be an application of using irreversible switches within genetic circuits  
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* Be an application of using irreversible switches within genetic circuits  
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4) Be an example of sensors being built into bacteria
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* Be an example of sensors being built into bacteria
==Background==
==Background==

Revision as of 02:40, 5 May 2009

Contents

20.109 Research Proposal for Derek Ju and Alvin Chen

Project Overview

Our goal is to rewire a bacterium to "miniprep" itself, when given some sort of an external stimuli (such as sensing of dense cell growth). This project will serve the following purposes:

  • Make the research process easier, cheaper, and more efficient for scientists working with bacteria.
  • Most laboratory strains have been genetically modified for scientific use; however our bacteria would be the first to be modified to do an experiment on itself.
  • Self-lysing of cells when dense cell growth is encountered - less chance for environmental contamination if genetically engineered bacteria escape from the lab
  • Serve as an example of a way synthetic biology can be used for cellular control
  • Be an application of using irreversible switches within genetic circuits
  • Be an example of sensors being built into bacteria

Background

Research Problems and Goals

Project Details and Methods

Predicted Outcomes

Resources Needed

References

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