| MODULE
| DAY
| DATE
| LECTURER
| TA
| LAB
| note
|
|
|
| Tue Feb 5
| ANS
| VL, BE
| Orientation
| Slides are here (warning: .ppt download)
|
|
|
| 1
| 1
| Thur Feb 7
| BE
| DW
| Day 1: DNA engineering using PCR
| PDF of slides is here
|
| 1
| 2
| Tue Feb 12
| BE
| DW
| Day 2: Clean and cut DNA
| PDF of slides is here
|
| 1
| 3
| Thur Feb 14
| BE
| DW
| Day 3: Agarose gel electrophoresis
| PDF of slides is here
|
|
|
| President's Day Feb 18
| No lab Feb 19 or Feb 20
|
| 1
| 4
| Thur Feb 21
| BE
| DW
| Day 4: DNA ligation and bacterial transformation
| PDF of slides is here
|
| 1
| 5
| Tue Feb 26
| BE
| DW
| Day 5: Examine candidate clones
| PDF of slides is here
|
| 1
| 6
| Thur Feb 28
| BE
| DW
| Day 6: Restriction map and tissue culture
| PDF of slides is here
|
| 1
| 7
| Tue Mar 4
| BE
| DW
| Day 7: Lipofection
| PDF of slides is here
|
| 1
| 8
| Thur Mar 6
| BE
| DW
| Day 8: FACS analysis
| PDF of slides is here
|
|
|
| 2
| 1
| Tue Mar 11
| AJ
| VL
| Day 1: Start-up protein engineering
| 'Introduction to the module' handout hardcopies are available, in class. (Ditto other Mod2 lectures.)
|
| 2
| 2
| Thur Mar 13
| AJ
| VL
| Day 2: Site-directed mutagenesis
| For some reason, the 'Rational protein design' handout is not available, despite past claims to the contrary.
|
| 2
| 3
| Tue Mar 18
| AJ
| VL
| Day 3: Prepare expression system
| For some reason, the 'Protein expression and purification' handout is not available, despite past claims to the contrary.
|
| 2
| 4
| Thur Mar 20
| AJ
| VL
| Day 4: Induce protein expression
| For some reason, the 'Binding and affinity measurements' handout is not available, despite past claims to the contrary.
|
|
|
| Spring Break Mar 24-28
| No lab all week
|
| 2
| 5
| Tue Apr 1
| AJ
| VL
| Day 5: Characterize protein expression
| For some reason, the 'Calcium sensors and sensing' handout is not available, despite past claims to the contrary.
|
| 2
| 6
| Thur Apr 3
| AJ
| VL
| Day 6: Assay protein behavior
| Please see Attisa's user page
|
| 2
| 7
| Tue Apr 8
| AJ
| VL
| Day 7: Data analysis
| 'High throughput screening' handout is available in class.
|
| 2
| 8
| Thur Apr 10
| AJ
| VL
| Day 8: Student presentations
| '"Real-world" protein engineering' handout is available in class.
|
|
|
| 3
| 1
| Tues Apr 15
| ANS
| BE
| Day 1: Start-up biomaterials engineering
| PDF of slides is here
|
| 3
| 2
| Thur Apr 17
| ANS
| BE
| Day 2: Initiate cell culture
| PDF of slides is here
|
|
|
| Patriot's Day April 21-22
| No lab Apr 22 or Apr 23
|
| 3
| 3
| Thur Apr 24
| ANS/NL
| BE
| Module 3 Day 3: Testing cell viability
| PDF of slides is here
|
| 3
| 4
| Tue Apr 29
| ANS
| BE
| Module 3 Day 4: Preparing cells for analysis
| PDF of slides is here
|
| 3
| 5
| Thur May 1
| ANS
| BE
| Module 3 Day 5: Transcript-level analysis
| PDF of slides is here
|
| 3
| 6
| Tue May 6
| ANS
| BE
| Module 3 Day 6: Protein-level analysis
| PDF of slides is here
|
| 3
| 7
| Thur May 8
| ANS
| BE
| Module 3 Day 7: Wrap-up analysis
| PDF of slides is here
|
| 3
| 8
| Tue May 13
| ANS
| BE
| Module 3 Day 8: Student presentations
| (Chalkboard lecture)
|
|
|
| Thur May 15
|
|
| No lab session. Evaluations during lecture, then celebratory lunch, place TBD.
|
|
