20.109(S08) - Revision history

AgiStachowiak: /* Announcements */

AgiStachowiak — Fri, 09 May 2008 17:04:42 GMT

Announcements

←Older revision		Revision as of 17:04, 9 May 2008
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	**last-minute q&a before research proposal presentations		**last-minute q&a before research proposal presentations

-	*~~'''~~On Thursday, May 15th during lecture we will have a discussion of the course followed by evaluations. This is an important chance to contribute your feedback.~~'''~~	+	*On Thursday, May 15th during lecture we will have a discussion of the course followed by evaluations. This is an important chance to contribute your feedback.

-	*~~'''~~On Thursday May 15th from ~12-1:30 pm, we will have a celebratory lunch in the Bush Room, 10-105.~~'''~~	+	*On Thursday May 15th from ~12-1:30 pm, we will have a celebratory lunch in the Bush Room, 10-105.

AgiStachowiak: /* Announcements */

AgiStachowiak — Fri, 09 May 2008 17:04:19 GMT

Announcements

←Older revision		Revision as of 17:04, 9 May 2008
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	*'''Module 3 presentations will begin at 1:30 pm, on both May 13th and 14th.'''		*'''Module 3 presentations will begin at 1:30 pm, on both May 13th and 14th.'''
-	**On Tuesday, presentations will be in 36-372.	+	**'''On Tuesday, presentations will be in 36-372.'''
-	**On Wednesday, presentations will be in 13-5101.	+	**'''On Wednesday, presentations will be in 13-5101.'''

	*Lecture on Tuesday, May 13th will consist of:		*Lecture on Tuesday, May 13th will consist of:
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	**short chalkboard lecture on drug delivery		**short chalkboard lecture on drug delivery
	**last-minute q&a before research proposal presentations		**last-minute q&a before research proposal presentations
-	*On Thursday, May 15th during lecture we will have a discussion of the course followed by evaluations.

-	*''On Thursday May 15th from ~12-1:30 pm, we will have a celebratory lunch in the Bush Room, 10-105.'''	+	*'''On Thursday, May 15th during lecture we will have a discussion of the course followed by evaluations. This is an important chance to contribute your feedback.'''
		+
		+	*'''On Thursday May 15th from ~12-1:30 pm, we will have a celebratory lunch in the Bush Room, 10-105.'''

AgiStachowiak: /* Announcements */

AgiStachowiak — Fri, 09 May 2008 17:02:41 GMT

Announcements

←Older revision		Revision as of 17:02, 9 May 2008
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	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]

-	*'''Module 2 presentations will ~~take place in 56-402~~, on both ~~April 10th~~ and ~~11th~~.'''	+	*'''Module 3 presentations will begin at 1:30 pm, on both May 13th and 14th.'''
		+	**On Tuesday, presentations will be in 36-372.
		+	**On Wednesday, presentations will be in 13-5101.

-	*~~In order to split your attention a little less drastically for next week~~, ~~and reduce the percentage~~ of ~~your assignments due all~~ in ~~one week~~, ~~the due date for Part 2~~ of the ~~portfolio (quasi~~-~~results+discussion) will be delayed to Day~~ 1 ~~of Module 3~~.	+	*Lecture on Tuesday, May 13th will consist of:
		+	**discussion of reasons for discrepancies in RT-PCR vs. ELISA data
		+	**short chalkboard lecture on drug delivery
		+	**last-minute q&a before research proposal presentations
		+	*On Thursday, May 15th during lecture we will have a discussion of the course followed by evaluations.
		+
		+	*''On Thursday May 15th from ~12-1:30 pm, we will have a celebratory lunch in the Bush Room, 10-105.'''

Atissa: /* Announcements */

Atissa — Tue, 06 May 2008 13:31:19 GMT

Announcements

←Older revision		Revision as of 13:31, 6 May 2008
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	*In order to split your attention a little less drastically for next week, and reduce the percentage of your assignments due all in one week, the due date for Part 2 of the portfolio (quasi-results+discussion) will be delayed to Day 1 of Module 3.		*In order to split your attention a little less drastically for next week, and reduce the percentage of your assignments due all in one week, the due date for Part 2 of the portfolio (quasi-results+discussion) will be delayed to Day 1 of Module 3.
-
-	*Please see [[User:Atissa \| Atissa's page]] for the schedule of presentation meetings.

Atissa: /* Announcements */

Atissa — Mon, 14 Apr 2008 18:45:08 GMT

Announcements

←Older revision		Revision as of 18:45, 14 April 2008
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	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]

-	*'''Module 2 presentations will take place in 56-402, on both April 10th and 11th.'''	+	*'''Module 2 presentations will take place in 56-402, on both April 10th and 11th.'''

	*In order to split your attention a little less drastically for next week, and reduce the percentage of your assignments due all in one week, the due date for Part 2 of the portfolio (quasi-results+discussion) will be delayed to Day 1 of Module 3.		*In order to split your attention a little less drastically for next week, and reduce the percentage of your assignments due all in one week, the due date for Part 2 of the portfolio (quasi-results+discussion) will be delayed to Day 1 of Module 3.
		+
		+	*Please see [[User:Atissa \| Atissa's page]] for the schedule of presentation meetings.

AgiStachowiak: /* Announcements */

AgiStachowiak — Wed, 02 Apr 2008 20:29:37 GMT

Announcements

←Older revision		Revision as of 20:29, 2 April 2008
Line 24:		Line 24:

	*'''Module 2 presentations will take place in 56-402, on both April 10th and 11th.'''		*'''Module 2 presentations will take place in 56-402, on both April 10th and 11th.'''
		+
		+	*In order to split your attention a little less drastically for next week, and reduce the percentage of your assignments due all in one week, the due date for Part 2 of the portfolio (quasi-results+discussion) will be delayed to Day 1 of Module 3.

AgiStachowiak: /* Announcements */

AgiStachowiak — Wed, 19 Mar 2008 14:48:29 GMT

Announcements

←Older revision		Revision as of 14:48, 19 March 2008
Line 23:		Line 23:
	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]

-	*Module 2 presentations will take place in 56-402, on both April 10th and 11th.	+	*'''Module 2 presentations will take place in 56-402, on both April 10th and 11th.'''

AgiStachowiak: /* Announcements */

AgiStachowiak — Wed, 19 Mar 2008 14:48:16 GMT

Announcements

←Older revision		Revision as of 14:48, 19 March 2008
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	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]
		+
		+	*Module 2 presentations will take place in 56-402, on both April 10th and 11th.

AgiStachowiak: /* Announcements */

AgiStachowiak — Mon, 17 Mar 2008 17:35:19 GMT

Announcements

←Older revision		Revision as of 17:35, 17 March 2008
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	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]
-
-	*'''Diagnostic digest results have been posted on the [[20.109%28S08%29:DNA_engineering/Restriction_map_and_tissue_culture_%28Day_6%29#Diagnostic_Digests\| Day 6]] "talk" page (02.29.08).'''
-
-	*A bit more Methods info (02.29.08):
-	**Gels were stained with ethidium bromide at a final concentration of 0.2 μg/mL.
-	**The E. coli cells for transformation were XL1-Blue cells from Stratagene.
-	**The ligase used was originally at 400,000 U/mL.
-
-	*A few pieces of information that may be useful for your Methods (posted 02.20.08):
-	**Gels this year were made in 1X TAE buffer, which contains 40 mM Tris (pH 8), 20 mM acetic acid, and 1 mM EDTA.
-	**The Qiagen kits you used are called the QIAquick PCR Purification Kit and the QIAquick Gel Extraction Kit. You may say that you used them according to the manufacturer’s instructions rather than describing the protocols step-by-step. However, you can briefly mention the principle components if you want (e.g., “DNA was purified on silica gel columns using the… kit and eluted in … buffer”).
-	**Your original digests (Day 2) contained ~ 5 μg of backbone. If you don’t know the amount or concentration of something (e.g., your PCR products), it’s fine to mention a volume instead in this case. Otherwise a final amount or concentration is generally preferred.
-	**Enzyme amounts are often given in terms of activity units, U. The XbaI and EcoRI enzyme stock solutions that you used are both at 20,000 U/mL.

AgiStachowiak: /* Announcements */

AgiStachowiak — Sat, 01 Mar 2008 04:00:52 GMT

Announcements

←Older revision		Revision as of 04:00, 1 March 2008
Line 23:		Line 23:
	*You can find old announcements [[20.109(S08): old announcements\| here]]		*You can find old announcements [[20.109(S08): old announcements\| here]]

-	*Diagnostic digest results have been posted on the [[20.109%28S08%29:DNA_engineering/Restriction_map_and_tissue_culture_%28Day_6%29#Diagnostic_Digests\| Day 6]] "talk" page (02.29.08).	+	*'''Diagnostic digest results have been posted on the [[20.109%28S08%29:DNA_engineering/Restriction_map_and_tissue_culture_%28Day_6%29#Diagnostic_Digests\| Day 6]] "talk" page (02.29.08).'''

	*A bit more Methods info (02.29.08):		*A bit more Methods info (02.29.08):