20.109(S14): Spring 2014 schedule
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| MODULE | DAY | DATE | LECTURER | TA | LAB | NOTES | |
| Tue/Wed Feb 4/5 | ANS | Chris | Orientation | Introductory Lecture (PDF) T/R Pre-Lab W/F Pre-Lab | |||
| 1 | 1 | Thur/Fri Feb 6/7 | JR | Chris | Microbial DNA extraction | lab orientation quiz Lecture L1 Notes T/R Pre-Lab W/F Pre-Lab | |
| 1 | 2 | Tue/Wed Feb 11/12 | JR | Chris | Diagnostic primer design | Lecture L2 Notes T/R Pre-lab W/F Pre-Lab | |
| 1 | 3 | Thur/Fri Feb 13/14 | JR | Chris | 16S PCR and paper discussion | lab quiz Lecture L3 Notes T/R Pre-lab W/F Pre-Lab WAC lecture, talks | |
| President's Day Feb 17 | No lab Feb 18 or Feb 19 | ||||||
|---|---|---|---|---|---|---|---|
| 1 | 4 | Thur/Fri Feb 20/21 | JR | Chris | DNA cloning | μg/mL L4 Notes T/R Pre-lab W/F Pre-Lab WAC lecture, figures | |
| 1 | 5 | Tue/Wed Feb 25/26 | JR | Chris | DNA sequencing | lab quiz | |
| 1 | 6 | Thur/Fri Feb 27/28 | JR | Chris | Journal club I | ||
| 1 | 7 | Tue/Wed Mar 4/5 | JR | Chris | Phylogenetic and primer analyses | ||
| 1 | 8 | Thur/Fri Mar 6/7 | JR | Chris | Journal club II | lab quiz | |
| 2 | 1 | Tue/Wed Mar 11/12 | LS | SV | Introduction to system components | Draft Mod 1 Abstract and Summary Due | |
| 2 | 2 | Thur/Fri Mar 13/14 | LS | SV | Cell plating and plasmid digestion | ||
| 2 | 3 | Tue/Wed Mar 18/19 | LS | SV | Begin Western protein analysis | Mod 1 Memo Due lab quiz | |
| 2 | 4 | Thur/Fri Mar 20/21 | LS | SV | Complete Western and plasmid purification | ||
| Spring Break Mar 24-28 | No lab all week | ||||||
| 2 | 5 | Tue/Wed Apr 1/2 | LS | SV | Cell preparation for DNA repair assays | lab quiz | |
| 2 | 6 | Thur/Fri Apr 3/4 | LS | SV | DNA repair assays | Final Mod 1 Abstract and Summary Due | |
| 2 | 7 | Tue/Wed Apr 8/9 | LS | SV | Data analysis | lab quiz | |
| 3 | 1 | Thur/Fri Apr 10/11 | ANS | RKA | Design experiment | ||
| 3 | 2 | Tue/Wed Apr 15/16 | ANS | RKA | Initiate cell culture | lab quiz | |
| 3 | 3 | Thur/Fri Apr 17/18 | ANS | RKA | Testing cell viability | ||
| Patriot's Day April 21-22 | No lab Apr 22 or Apr 23 Mod 2 Report Due |
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| 3 | 4 | Thu/Fri Apr 24/25 | ANS | RKA | Preparing cells for analysis | lab quiz | |
| Tue/Wed Apr 29/30 | No lecture or lab today. | ||||||
| 3 | 5 | Thur/Fri May 1/2 | ANS | RKA | Initiating transcript and protein assays | ||
| 3 | 6 | Tue/Wed May 6/7 | ANS | RKA | Transcript-level analysis | lab quiz | |
| 3 | 7 | Thur/Fri May 8/9 | ANS | RKA | Protein-level and wrap-up analysis | Mod 3 Mini-report Due | |
| 3 | 8 | Tue/Wed May 13/14 | ANS | RKA | Student presentations | ||
| Thur May 15 | No lab session. Meet in TBD from 11am-1pm: feedback/discussion about class, then celebratory lunch etc. | ||||||
