20.109(S14):Introduction to cell strains and plating (Day1)
Introduction
Topic 1: module overview
Topic 2: tissue culture
Protocols
Part 1: WAC discussion about research article organization
Leslie will join us first thing today to talk about the structure and components of a research article in preparation for the major communication assessment for Module 2.
Part 2: Introduction to tissue culture
We'll then continue with pre-lab lecture: a brief overview of the Module 2 experiment and a discussion of concepts and pragmatics related doing tissue culture.
Afterward, half of the class will begin with Part 3 and half will begin with Part 4 below, since we cannot all fit in the tissue culture room at once.
Part 3 (or 4): Plating cells for next time
We will begin with a brief demo about sterile technique and how to use the tissue culture hoods.
Overview
Each team will be given two T25 culture flasks: one with CHO-K1 cells, and one with xrs6 cells. You will enzymatically detach these adherent cells, count them, and plate them at a specific seeding density in a 6-well culture plate. Next time, you will lyse these very cells and begin your first assay of the module. For this reason, it is important that you take care to use sterile technique today.
It is essential that you do not mix up or cross-contaminate the K1 and xrs6 cell lines! We suggest that one partner is responsible for each flask, and that partners do not share Pasteur pipets or other equipment.
Protocol
Part 4 (or 3): Getting familiar with Module 2 cell lines
For next time
Reagent list
write something here or not accessible to edit
Next Day: Begin Western protein analysis
