20.109(S14):Begin Western protein analysis (Day2)
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Introduction
Topic 1: Reiterate purpose of WB, now some details about cell lines
Topic 2: Basis of WB, including background on antibodies
Protocols
Part 1: Prepare cell lysates
Part 2: Measure protein content
Part 3: Separate proteins by SDS-PAGE
Part 4: Transfer proteins to membrane
For next time
Reagent list
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Next Day: Choose system conditions and paper discussion Previous Day: Introduction to cell strains and plating