20.109(S13): Primer design summary

Overview

You will write a brief memo about your study of diagnostic primers for identifying microsporidia.

The target audience is an advisor for whom you are preparing a relatively informal (but accurate!) report.

Logistics

Please email your completed summary to 20109 DOT submit AT gmail DOT com, with filename Firstinitial_Lastname_LabSection_Mod1.doc (for example, R_Reif_TR_Mod2.doc).

This assignment is due by 11 am on March 19th (Tuesday) or March 10th (Wednesday), according to which day you have lab.

Content Guidelines

Two primary sections -- one on design and one on results -- will make up the memo.

Design

Here you should include the following:

• A short (1-2 paragraph) introduction motivating your design challenge and explaining your design choices.
• A table comparing the properties of the V1-PMP2 primer set and your primer set.

The text related to your specific design choices should explicitly refer to the table.

Results

Here you should include the following:

• A short (1-2 paragraph) description of your findings.
• A figure depicting your raw PCR results (as seen on a gel).
• Optionally, a figure or table depicting the PCR results in some processed form.

The figure should include a complete caption as for a publication.