20.109(S13): Final Project WFRedBlue: Difference between revisions
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== W/F Red/Blue Team Final Project == | == W/F Red/Blue Team Final Project == | ||
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== Topic and Focus == | == Topic and Focus == | ||
""Topic:"" Cellular Programming and Biosensors | ""Topic:"" Cellular Programming and Biosensors | ||
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==Background== | ==Background== | ||
1. http://www.nature.com/nature/journal/vaop/ncurrent/full/nature11149.html | ''1. http://www.nature.com/nature/journal/vaop/ncurrent/full/nature11149.html'' | ||
- Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder | - Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder | ||
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- Goal: Perform therapeutic calculations in animal metabolism | - Goal: Perform therapeutic calculations in animal metabolism | ||
2. Synthetic circuits integrating logic and memory in living cells[1] | ''2. Synthetic circuits integrating logic and memory in living cells[1]'' | ||
In order to generate complex, state-dependent responses, it is necessary to be able to incorporate logic functions and memory stores. In this study, researchers used recombinases to create Boolean logic functions and DNA-encoded memory on live E. Coli cells. The purpose of this is to be able to create cellular networks capable of producing certain responses given input signals. Furthermore, DNA storage could present a new way of long-term storage, due to the cells self propagation and the stability of DNA. | In order to generate complex, state-dependent responses, it is necessary to be able to incorporate logic functions and memory stores. In this study, researchers used recombinases to create Boolean logic functions and DNA-encoded memory on live E. Coli cells. The purpose of this is to be able to create cellular networks capable of producing certain responses given input signals. Furthermore, DNA storage could present a new way of long-term storage, due to the cells self propagation and the stability of DNA. | ||
Siuti, P., Yazbek, J., & Lu, T. K. (2013). Synthetic circuits integrating logic and memory in living cells. Nature Biotechnology, 1–6. doi:10.1038/nbt.2510 | |||
''3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564264/'' | |||
Details/Methods | == Details/Methods == | ||
- Fluorescence Assay | - Fluorescence Assay | ||
- Cell Viability Assay | - Cell Viability Assay | ||
Predicted Outcomes | |||
== Predicted Outcomes == | |||
- Engineered tunability of logic gate sensitivity to threshold concentrations of an input substance | - Engineered tunability of logic gate sensitivity to threshold concentrations of an input substance | ||
Necessary Resources: | |||
== Necessary Resources: == |
Revision as of 09:47, 8 May 2013
W/F Red/Blue Team Final Project
Topic and Focus
""Topic:"" Cellular Programming and Biosensors ""Specific focus:"" Using biosensors to refine and amplify cellular logic gates
Background
1. http://www.nature.com/nature/journal/vaop/ncurrent/full/nature11149.html
- Implements boolean logic gates: NAND, XOR, and N-IMPLY, half-subtractor, half-adder - Gradual disassembly of transcriptional / translational components exhibited characteristics of simpler logic gates - Individual components can be rewired into combinatorial structures (modular) - Novelty: all components of logic gates contained in one cell (opposed to others which incorporate intercellular signaling) - Goal: Perform therapeutic calculations in animal metabolism
2. Synthetic circuits integrating logic and memory in living cells[1]
In order to generate complex, state-dependent responses, it is necessary to be able to incorporate logic functions and memory stores. In this study, researchers used recombinases to create Boolean logic functions and DNA-encoded memory on live E. Coli cells. The purpose of this is to be able to create cellular networks capable of producing certain responses given input signals. Furthermore, DNA storage could present a new way of long-term storage, due to the cells self propagation and the stability of DNA.
Siuti, P., Yazbek, J., & Lu, T. K. (2013). Synthetic circuits integrating logic and memory in living cells. Nature Biotechnology, 1–6. doi:10.1038/nbt.2510
3. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564264/
Details/Methods
- Fluorescence Assay - Cell Viability Assay
Predicted Outcomes
- Engineered tunability of logic gate sensitivity to threshold concentrations of an input substance